{"id":31,"date":"2016-03-17T02:26:26","date_gmt":"2016-03-17T01:26:26","guid":{"rendered":"http:\/\/www.itpa.it\/?p=31"},"modified":"2019-03-17T15:04:15","modified_gmt":"2019-03-17T14:04:15","slug":"experimental-setup-for-the-identification-of-mitochondrial-protease-substrates-by-shotgun-and-top-down-proteomics","status":"publish","type":"post","link":"https:\/\/www.itpa.it\/index.php\/publications\/experimental-setup-for-the-identification-of-mitochondrial-protease-substrates-by-shotgun-and-top-down-proteomics\/","title":{"rendered":"Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics"},"content":{"rendered":"<p><img decoding=\"async\" class=\"alignnone size-full wp-image-32\" src=\"https:\/\/www.itpa.it\/wp-content\/uploads\/bondi_et_al.jpg\" alt=\"\" width=\"100%\" srcset=\"https:\/\/www.itpa.it\/wp-content\/uploads\/bondi_et_al.jpg 500w, https:\/\/www.itpa.it\/wp-content\/uploads\/bondi_et_al-300x120.jpg 300w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/p>\n<p style=\"text-align: justify;\">Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences<\/p>\n<p>&nbsp;<\/p>\n<p><strong><a href=\"https:\/\/www.itpa.it\/uploads\/2016_di_pierro_et_al_eupaopen\/\">2016 di pierro et al eupaopen<\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of&#46;&#46;&#46;<\/p>\n","protected":false},"author":1,"featured_media":32,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[3],"tags":[],"class_list":["post-31","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"_links":{"self":[{"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/posts\/31","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/comments?post=31"}],"version-history":[{"count":7,"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/posts\/31\/revisions"}],"predecessor-version":[{"id":89,"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/posts\/31\/revisions\/89"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/media\/32"}],"wp:attachment":[{"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/media?parent=31"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/categories?post=31"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.itpa.it\/index.php\/wp-json\/wp\/v2\/tags?post=31"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}